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Following purification, the protein was diluted to 0.08 mg/mL in refolding buffer at pH 8.5 (10.56 mM NaCl, 55 mM Tris-HCl, 2.2 mM CaCl2, 2.2 mM MgCl2, 0.055% PEG4000, 0.55 M L-arginine, 0.1 mM oxidized glutathione, and 1 mM reduced glutathione) and incubated overnight at 4C with shaking

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However, it showed no significant effect on belly fat or overall body fat percentage

Suppressing hunger Both Ozempic and Wegovy are produced by Danish company Novo Nordisk, and whose active ingredient is semaglutide, says David Stensel, professor of exercise metabolism at Loughborough University